FluoSite™ Anti-STEAP1 Antibody, FITC Labeled

This anti-STEAP1 (six transmembrane epithelial antigen of the prostate 1) antibody is a purified monospecific recombinant antibody, which is labeled with Fluorescein isothiocyanate (FITC) . This antibody has been tested by flow cytometry for binding to human STEAP1 on the surface of STEAP1 HEK293 Cell Line (BPS Bioscience #82749) .FluoSite™is a tag-based method for site-specific conjugation of antibodies with fluorophores, ensuring the labeling site is far from the antigen-binding domain to preserve antibody functionality. This precise, consistent labeling ensures the homogeneity of the labeled antibody preparation, making it ideal for flow cytometry and other high-sensitivity applications.

Product Specifications

Background

STEAP1 (six transmembrane epithelial antigen of the prostate 1), also called STEAP, belongs to the STEAP family of metalloproteinases, which are involved in iron and copper regulation and other processes. It is a cell surface protein expressed in several types of cancer, most notably prostate cancer where STEAP1 was identified as a cancer specific cell-surface antigen. The upregulation of STEAP1 is linked to promotion of cancer cell proliferation and metastatic transformation and higher STEAP expression levels are correlated with poor survival. The differential expression of STEAP1 between cancer cells and normal tissue makes STEAP1 a promising target for antibody-drug-conjugate (ADC) development. The use of STEAP1 CAR (chimeric antigen receptor) -T cell therapy combined with tumor-localized IL-12 therapy is also under study for the treatment of prostate cancer.

UniProt

Q9UHE8

Cross Reactivity

This antibody recognizes human STEAP1. It has not been tested in other species.

Label

Fluorescein isothiocyanate (FITC) is a derivative of fluorescein and has an excitation and emission spectrum peak wavelengths of approximately 495 nm and 519 nm, causing it to fluoresce green.

Applications

Useful for studying the expression of human STEAP1 on the cell surface by flow cytometry.

Purification

Protein A affinity chromatography from HEK293 supernatant.

Purity

≥ 90%

Format

Aqueous buffer solution.

Shipping Conditions

+4°C (Gel Packs)

Storage Conditions

At least 6 months at +4°C. Protect from prolonged exposure to light. DO NOT FREEZE. The antibody solution should be stored undiluted between +2°C and +8°C.