FluoSite™ Anti-CD4 Antibody, PE-Labeled

This anti-CD4 antibody is a purified recombinant antibody, which is labeled with R-Phycoerythrin (PE) . This antibody has been tested by flow cytometry for specific binding to human CD4 on the surface of PBMCs (BPS Bioscience #79059) .FluoSite™is a tag-based method for site-specific conjugation of antibodies with fluorophores, ensuring the labeling site is far from the antigen-binding domain to preserve antibody functionality. This precise, consistent labeling ensures the homogeneity of the labeled antibody preparation, making it ideal for flow cytometry and other high-sensitivity applications.

Product Specifications

Background

CD4 (cluster of differentiation 4) is part of the immunoglobulin superfamily, and is found in T-helper cells, monocytes, macrophages and dendritic cells. It is a co-receptor in the TCR (T-cell receptor), binding to MHC (major histocompatibility complex) class II molecules. CD4 binds to the tyrosine kinase Lck (lymphocyte-specific protein tyrosine kinase), which can phosphorylate the ITAM (immunoreceptor tyrosine activation motifs) domain of the CD3, activating CD3-related signaling. CD4 is a typical T cell marker of T helper cells, and it has been linked to cancer, autoimmune diseases such as vitiligo and type I diabetes. In addition, HIV-1 makes use of CD4 to trigger viral envelope protein conformational changes that allow cell infection. Ibalizumab, an anti-CD4 antibody, is currently used in the treatment of HIV, being considered a first-in-class medication. Further studies into CD4 and potential strategies around it may benefit patients and CD4 related diseases.

Cross Reactivity

This antibody recognizes human CD4. It has not been tested on other species.

Label

PE is an oligomeric protein complex (270 kDa) from red algae that exhibits intensely bright red-orange fluorescence with excitation peak at 566 nm and an emission peak at 574 nm. The complex consists of six heterodimers, α subunit (18 kDa) and β-subunit (20 kDa), and an additional γ-subunit (34 kDa) .

Applications

Useful for studying expression of CD4 on the cell surface by flow cytometry.

Purification

Protein A affinity chromatography from HEK293 supernatants.

Purity

≥ 90%

Shipping Conditions

+4°C (Gel Packs)

Storage Conditions

At least 6 months at 4°C. Protect from prolonged exposure to light. DO NOT FREEZE. The antibody solution should be stored undiluted between 2°C and 8°C.