FluoSite™ Anti-CD16a Antibody, FITC-Labeled

This human anti-CD16a antibody is a purified recombinant antibody, which is labeled with fluorescein isothiocyanate (FITC) . This antibody has been tested by flow cytometry for binding to human CD16a on the surface of the CD16a CHO Recombinant Cell Line (BPS Bioscience #78332) .FluoSite™is a tag-based method for site-specific conjugation of antibodies with fluorophores, ensuring the labeling site is far from the antigen-binding domain to preserve antibody functionality. This precise, consistent labeling ensures the homogeneity of the labeled antibody preparation, making it ideal for flow cytometry and other high-sensitivity applications.

Product Specifications

Background

CD16a, also known as Fc Gamma Receptor 3A (FcGRIIIA; FcγRIIIA), is a low/intermediate affinity receptor for polyvalent immune-complexed IgG. It is involved in phagocytosis, antibody-dependent cytotoxicity, and clearance of immune complexes. The human FcGR3A displays a dimorphism at residue 158. The allele V158 encodes a higher Fc affinity receptor variant while the allele F158 encodes a lower Fc affinity receptor variant. FcGR3A (CD16a) plays a role in the activation of natural killer (NK) cells, and it is extensively used as a cell marker. Its expression has also been observed at the surface of T cells in patients with chronic viral infections (such as COVID-19) . Binding to CD16a can potentiate the efficacy of therapeutic antibodies used to treat solid tumors. In addition, CD16a is a biomarker in hematopoietic cancers and an interesting therapeutic target.

UniProt

P08637

Cross Reactivity

This antibody recognizes human CD16a. It has not been tested on other species

Label

Fluorescein isothiocyanate (FITC) is a derivative of fluorescein and has excitation and emission spectrum peak wavelengths of approximately 495 nm and 519 nm, causing it to fluoresce green.

Applications

Useful for studying expression of CD16a on the cell surface by flow cytometry. For flow cytometric staining, the suggested use for this reagent is 5 µl (1 µg antibody) per million cells in 100 µl of staining volume

Purification

Protein A affinity chromatography from HEK293 supernatant

Purity

≥ 90%

Format

Aqueous buffer solution.

Shipping Conditions

+4°C (Gel Packs)

Storage Conditions

At least 6 months at 4°C. Protect from prolonged exposure to light. DO NOT FREEZE. The antibody solution should be stored undiluted between 2°C and 8°C.