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FluoSite™ Anti-NCAM1 Antibody, FITC-Labeled

This anti-NCAM1 antibody is a purified recombinant antibody, which is labeled with fluorescein isothiocyanate (FITC) . This antibody has been tested by flow cytometry for specific binding to human NCAM1 on the surface of NCAM1 CHO Recombinant Cell Line (BPS Bioscience #78352) .FluoSite™is a tag-based method for site-specific conjugation of antibodies with fluorophores, ensuring the labeling site is far from the antigen-binding domain to preserve antibody functionality. This precise, consistent labeling ensures the homogeneity of the labeled antibody preparation, making it ideal for flow cytometry and other high-sensitivity applications.

Product Specifications

Background

NCAM1 (Neural cell adhesion molecule 1), also known as CD56 (Cluster of Differentiation 56), is a cell surface glycoprotein expressed typically in NK (Natural Killer) cells. Its level correlates with the cytotoxicity potential of the NK cells. Deficiencies in CD56+immune cells have been associated with specific infections and autoimmune diseases. NCAM1 expression is altered in hematological malignancies, such as multiple myeloma and leukemia, and solid tumor cells from various tissue types. Studies of NCAM1 function and expression patterns will continue to broaden our knowledge and support possible applications in cancer therapy.

UniProt

P13591

Cross Reactivity

This antibody recognizes human NCAM1. It has not been tested in other species.

Label

Fluorescein isothiocyanate (FITC) is a derivative of fluorescein and has excitation and emission spectrum peak wavelengths of approximately 495 nm and 519 nm, causing it to fluoresce green.

Applications

Useful for studying expression of NCAM1 on the cell surface by flow cytometry. For flow cytometric staining, the suggested use of this reagent is 5 µl (1 µg antibody) per million cells in 100 µl staining volume.

Purification

Protein A affinity chromatography from HEK293 supernatant.

Purity

≥ 90%

Format

Aqueous buffer solution.

Shipping Conditions

+4°C (Gel Packs)

Storage Conditions

At least 6 months at 4°C. Protect from prolonged exposure to light. DO NOT FREEZE. The antibody solution should be stored undiluted between 2°C and 8°C.

Formulation

8 mM Phosphate, pH 7.4,110 mM NaCl, 2.2 mM KCl, 0.09% Sodium Azide, 0.2% BSA, and up to 20% glycerol. May contain a protein stabilizer.

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