FluoSite™ Anti-CD20 Antibody, FITC-Labeled

This anti-CD20 antibody is purified recombinant antibody, which is labeled with fluorescein isothiocyanate (FITC) . This antibody has been tested by flow cytometry for specific binding to human CD20 on the surface of CD20 CHO Recombinant Cell Line (BPS Bioscience #79624) . FluoSite™ is a tag-based method for site-specific conjugation of antibodies with fluorophores, ensuring the labeling site is far from the antigen-binding domain to preserve antibody functionality. This precise, consistent labeling ensures the homogeneity of the labeled antibody preparation, making it ideal for flow cytometry and other high-sensitivity applications.

Product Specifications

Background

CD20 (also known as MS4A1) is a glycosylated phosphoprotein expressed on the cell surface of B cells. CD20 is a highly attractive target antigen for immunotherapy because it is highly expressed in more than 90% of patients with B-cell lymphoma, but not in the first stage of B cell development. First approved in 1997, Rituximab (Rituxan) is a chimeric monoclonal antibody targeting CD20 and has been classified by the World Health Organization as an “Essential Medicine”. Since then, additional monoclonal antibodies against CD20 have been approved or are being tested in clinical trials for the treatment of multiple sclerosis (MS), chronic lymphocytic leukemia (CLL), follicular lymphoma, rheumatoid arthritis, non-Hodgkin’s lymphoma, systemic lupus erythematosus, and other inflammatory diseases and cancer types. Studies of CD20 function and expression patterns will continue to broaden our knowledge and application in cancer therapy.

UniProt

P11836

Cross Reactivity

This antibody recognizes human CD20. It has not been tested on other species.

Label

Fluorescein isothiocyanate (FITC) is a derivative of fluorescein and has excitation and emission spectrum peak wavelengths of approximately 495 nm and 519 nm, causing it to fluoresce green.

Applications

Useful for studying expression of CD20 on the cell surface by flow cytometry.For flow cytometric staining, the suggested use of this reagent is 5 µl (1µg antibody) per million cells in 100 µl staining volume.

Purification

Protein A affinity chromatography from HEK293 supernatant.

Purity

≥ 90%

Format

Aqueous buffer solution.

Shipping Conditions

+4°C (Gel Packs)

Storage Conditions

At least 6 months at 4°C. Protect from prolonged exposure to light. DO NOT FREEZE. The antibody solution should be stored undiluted between 2°C and 8°C.