FluoSite™ Anti-CD235a Antibody, PE-Labeled

This anti-CD235a antibody is a purified recombinant antibody, which is labeled with R-Phycoerythrin (PE) . This antibody has been tested in flow cytometry assay for specific binding to human CD235a on the surface of CD235a CHO Recombinant Cell Line (BPS Bioscience #78353) .FluoSite™is a tag-based method for site-specific conjugation of antibodies with fluorophores, ensuring the labeling site is far from the antigen-binding domain to preserve antibody functionality. This precise, consistent labeling ensures the homogeneity of the labeled antibody preparation, making it ideal for flow cytometry and other high-sensitivity applications.

Product Specifications

UniProt

P02724

Cross Reactivity

This antibody recognizes human CD235a. It has not been tested with other species.

Label

PE is an oligomeric protein complex (270 kDa) from red algae that exhibits intensely bright red-orange fluorescence with excitation peak at 566 nm and an emission peak at 574 nm. The complex consists of six heterodimers, α subunit (18 kDa) and β-subunit (20 kDa), and an additional γ-subunit (34 kDa) .

Applications

Useful for studying expression of CD235a on the cell surface in flow cytometry assay. For flow cytometric staining, the suggested use of this reagent is 5 µl (1 µg antibody) per million cells in 100 µl staining volume.

Purification

Protein A affinity chromatography from HEK293 supernatants.

Concentration

The concentration will vary with each lot; the exact value will be provided with each shipment.

Purity

≥ 90%

Format

Aqueous buffer solution

Shipping Conditions

+4°C (Gel Packs)

Storage Conditions

At least 6 months at 4°C. Protect from prolonged exposure to light. DO NOT FREEZE. The antibody solution should be stored undiluted between 2°C and 8°C.