KRAS (G12D), Isoform A, His-Tag, GDP-Loaded Recombinant

Recombinant human KRAS (GTPase), isoform a, encompassing amino acids 2-186 (end) . This construct contains an N-terminal His-tag (6xHis) followed by an optimal TEV protease target sequence that allows cleavage of the His-tag from the protein (TEV: tobacco etch virus cysteine protease, BPS Bioscience #50308) . The recombinant protein also contains a mutation of interest, G12D. The protein was affinity purified and loaded with GDP. Unbound GDP was removed by spin column.

Product Specifications

Background

RAS mutations are responsible for more than 30% of human cancers. KRAS (G12D) is one of the KRAS mutations that is found frequently in pancreatic and colon cancers. Recent studies have led to the discovery of a small molecule called MRTX-1133 able to lock KRAS conformation in the inactive GDP-bound state, thereby blocking the KRAS (G12D) -mediated signaling pathway. Compounds that affect the nucleotide exchange (GDP to GTP) reaction in KRAS may inhibit tumor cell growth in KRAS (G12D) -driven tumors. Further studies into KRAS (G12D) mode of action and specific inhibitors will open new avenues in cancer therapy.

UniProt

P01116

Tag

N-terminal His-tag

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Purity

≥ 90%

Shipping Conditions

-80°C

Storage Conditions

At least 6 months at -80°C.

Calculated Molecular Weight

23 kDa

Formulation

20 mM HEPES, pH 7.4,150 mM NaCl, and 1 mM DTT