KRAS (G12D), Isoform A, His-Tag, GDP-Loaded Recombinant
Recombinant human KRAS (GTPase), isoform a, encompassing amino acids 2-186 (end) . This construct contains an N-terminal His-tag (6xHis) followed by an optimal TEV protease target sequence that allows cleavage of the His-tag from the protein (TEV: tobacco etch virus cysteine protease, BPS Bioscience #50308) . The recombinant protein also contains a mutation of interest, G12D. The protein was affinity purified and loaded with GDP. Unbound GDP was removed by spin column.
Product Specifications
Background
UniProt
P01116
Tag
N-terminal His-tag
Applications
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Purity
≥ 90%
Shipping Conditions
-80°C
Storage Conditions
At least 6 months at -80°C.
Calculated Molecular Weight
23 kDa
Formulation
20 mM HEPES, pH 7.4,150 mM NaCl, and 1 mM DTT
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