IL-17RA:IL-17A[Biotinylated] Inhibitor Screening Assay Kit

IL-17 (interleukin 17) is a cytokine involved in inflammation and belongs to the pro-inflammatory cystine knot cytokine family. The IL-17 family includes IL-17A-F. It binds to the receptor IL-17R, which has three variants IL-17RA-C, in T helper 17 (Th17) cells in response to IL-23. It is also produced by macrophages, dendritic cells and δγ T cells. Activation of downstream pathways leads to the release of chemokines, which can recruit immune cells to inflammation sites, cytokines (such as IL-6 and GCSF (granulocyte colony stimulating factor) ) and complement proteins. IL17A is also involved in differentiation of CD34+ hematopoietic progenitor cells into neutrophils. Higher levels IL17A have been linked to autoimmune disorders such as RA (rheumatoid arthritis), lupus psoriasis and asthma, transplant rejection and MS (multiple sclerosis) . Inhibitors of IL-17 have been under development, as treatment options for autoimmune diseases, with the first monoclonal antibody been approved by the FDA in 2015 for the treatment of plaque psoriasis (secukinumab, sold under the commercial name of Cosentyx) . The success of these drugs indicates the relevance of this cytokine in human health and disease, making it a valuable therapeutic target.

96 WellCatalog #ComponentAmountStorage11264IL-17RA, Fc fusion, Avi-Tag*10 µg-80°CIL-17A, Biotin*1 µg-80°C793113x Immuno Buffer 150 ml-20°CBlocking Buffer 750 ml+4°C79742Streptavidin-HRP10 µl+4°C79670ELISA ECL substrate A (translucent bottle) 6 mlRoom TempELISA ECL substrate B (brown bottle) 6 mlRoom Temp79699White 96-well microplate1Room Temp*The concentration of the protein is lot-specific and will be indicated on the tube.384 WellCatalog #NameAmountStorage11264IL-17RA, Fc fusion, Avi-Tag*2x 10 µg-80°CIL-17A, Biotin Labeled*2x 1 µg-80°C793113x Immuno Buffer 150 ml-20°C82719Blocking Buffer 72 x 50 ml+4°C79742Streptavidin HRP2x 10 µl+4°C79670ELISA ECL Substrate A (translucent bottle) 2x 6 mlRoom TempELISA ECL Substrate B (brown bottle) 2x 6 mlRoom Temp78188White 384-well microplate1Room Temp*The concentration of the protein is lot-specific and will be indicated on the tube.

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