Diablo, His-Tag Recombinant
Recombinant human Diablo (direct IAP binding protein with low pI), transcript 1, encompassing amino acids 56-239 (end) . This construct contains a C-terminal His-tag (6xHis) . This protein was affinity purified.
Product Specifications
Background
Diablo (direct IAP binding protein with low pI), also named SMAC (second mitochondria-derived activator of caspases), is a ubiquitously expressed mitochondrial protein involved in apoptosis. Under normal conditions this protein is found in the intermembrane space of the mitochondria. However, when a cell undergoes apoptosis, the mitochondrial membrane becomes porous and Diablo moves the cytosol. Once in the cytosol it can bind and inhibit IAPs (inhibitor of apoptosis protein) proteins, allowing caspase activation. This protein has been linked to cancer, and overexpression of Diablo may sensitize cancer cells to apoptosis. The use of Diablo mimetics, alone or in combination with other therapeutical approaches, is a promising cancer therapy.
UniProt
Q9NR28
Tag
C-terminal His-tag
Applications
Useful for binding assays with XIAP.
Purity
≥ 90%
Format
Aqueous buffer solution.
Shipping Conditions
-80°C
Storage Conditions
At least 6 months at -80°C.
Calculated Molecular Weight
22 kDa
Formulation
40 mM Tris-HCl, pH 8.0,110 mM NaCl, 2.2 mM KCl, 20% glycerol, and variable Imidazole
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