Membrane-Bound TNFα Jurkat Cell Line

Tumor necrosis factor (TNF, also known as TNFα) is a cytokine produced predominantly by activated macrophages and T lymphocytes. It has been identified as a key regulator in inflammatory and immune responses. TNF signaling pathways are triggered by binding to one of two distinct receptors, designated TNFR1 (TNF receptor 1) and TNFR2, which are differentially regulated on various cell types in normal and diseased tissues. TNFα exists in both a trimeric membrane-bound form (mTNFα) and as a soluble protein. TNFα is synthetized in a precursor form, a cell surface type II transmembrane protein, which is cleaved by metalloproteinases such as TACE (TNFα converting enzyme) into a soluble peptide. Soluble TNFα can then bind to its receptors and activate downstream signaling pathways. Transmembrane TNFα can also bind to TNFα receptors and induce cellular responses. For instance, it can enhance cytotoxicity in NK cells, while in the liver it can trigger hepatitis. Anti-TNFα antibodies can bind to mTNFα and trigger antibody-dependent cell-mediated cytotoxicity (ADCC) or complement-dependent cytotoxicity (CDC) to destroy the mTNFα-expressing inflammatory cells, being a promising therapy for inflammatory diseases.

Screen and validate antibodies against mTNFα.Useful as target cells to measure ADCC (antibody dependent cellular cytotoxicity) or CDC (complement-dependent cytotoxicity) responses to anti-TNFα antibodies.

Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

License DisclosureVisitbpsbioscience.com/licensefor the label license and other key information about this product.Troubleshooting GuideVisitbpsbioscience.com/cell-line-faqfor detailed troubleshooting instructions. For all further questions, please email[email protected].