Chemi-Verse™ CDK5/p25 Kinase Assay Kit

CDK5 (cyclin dependent kinase 5) is a serine/threonine protein kinase of the CDK family of proteins. It is expressed mainly in the brain, where it is linked to neuronal development and differentiation. CDK5 is an unconventional CDK protein, as its partners are the non-cyclin proteins p35 (early 35 kDa protein) and p39. p35 and p39 are myristoylated and recruit CDK5 to the cell membrane. Recent findings identified new roles for CDK5 in the nervous system, such as participating in neurite outgrowth, synaptic plasticity and homeostasis and circadian rhythm. Accumulation of p25, a fragment of p35, in neurons creates dysregulation of CDK5 function, and can result in Alzheimer’s disease (AD), Parkinson’s disease (PD), ALS (amyothropic lateral sclerosis) and Huntington’s disease (HD) . CDK5 also participates in T cell activation, and overexpression of this protein has been found in cancer samples. It acts on increasing brain tumor stem cell numbers and can lead to upregulation of PD-L1 (programmed death ligand-1) and increase cancer cell immune evasion. Inhibition of CDK5 via small molecules and peptides is a promising area of research that can benefit patients with cancer with neurodegenerative disorders.

Catalog #NameAmountStorage40105CDK5/p25, GST-tag*>1 µg-80°C793345x Kinase Assay Buffer 11.5 ml-20°C79686500 µM ATP50 µl-20°C7959810x CDK substrate peptide 1250 µl-20°C79696White 96-well plate1Room Temperature*The concentration of the protein is lot-specific and will be indicated on the tube.

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