CD200R1:CD200 [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit

The CD200R1:CD200 [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit is an ELISA designed to assess the efficacy of inhibitors of the binding between CD200R1 (CD200 receptor 1) and CD200 (cluster of differentiation 200) protein for screening and profiling applications. This kit comes with enough purified CD200R1 (amino acids 29-265) and biotinylated CD200 (amino acids 31-232), streptavidin-HRP, assay buffer, and detection reagent for 100 enzyme reactions.Figure 1. CD200R1:CD200 [Biotinylated] Inhibitor Screening ChemiluminescenceAssay Kit schematic.A 96-well plate is coated with CD200R1 protein. After blocking, biotinylated CD200 is added in an optimized assay buffer. Next, unbound biotinylated CD200 is washed away, and the plate is incubated with streptavidin-HRP. After a final wash, ELISA ECL substrate is added to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to CD200 binding to CD200R1.Need us to run inhibitor screens or profile your compounds against IL-17RA:IL-17A[Biotin]? Check out ourImmunotherapy Biochemical Screening Services.

Product Specifications

Background

CD200 (Cluster of Differentiation 200) and its receptor, CD200R, are cell surface proteins that play a crucial role in immune regulation and tolerance. The CD200-CD200R pathway is primarily involved in inhibiting immune responses, helping to maintain immune homeostasis and prevent excessive inflammation. In many cancers, tumor cells overexpress CD200, which interacts with CD200R on immune cells, particularly myeloid cells like macrophages and dendritic cells. This interaction leads to the suppression of immune responses against the tumor, effectively promoting immune evasion. Neutralizing antibodies against CD200 or CD200R can block this interaction, preventing the inhibitory signal from being delivered to immune cells. As a result, the anti-tumor immune response is enhanced, leading to improved recognition and elimination of tumor cells by the immune system. The development of strategies targeting this complex holds great potential in cancer therapy.

UniProt

Q8TD46, P41217

Applications

Study and screen compounds that inhibit the binding of CD200R1 to CD200 for drug discovery in high throughput screening (HTS) applications.

Format

Catalog #NameAmountStorage102034CD200R1, His-Tag*5 µg-80°C102014CD200, Fc Fusion, Avi-Tag, Biotin-Labeled Recombinant*> 0.5 µg-80°C826205x PP-02 Buffer4 ml-20°C79743Blocking Buffer 350 ml+4°C79742Streptavidin HRP10 µl+4°C79670ELISA ECL Substrate A (translucent bottle) ELISA ECL Substrate B (brown bottle) 6 mlRoom Temp79699White 96-well microplate1Room Temp*The concentration of the protein is lot-specific and will be indicated on the tube.

Shipping Conditions

-80°C

Storage Conditions

This assay kit will perform optimally for up to6 monthsfrom date of receipt when the materials are stored as directed.

Notes

Troubleshooting GuideVisitbpsbioscience.com/assay-kits-faqfor detailed troubleshooting instructions. For all further questions, please email[email protected]