Activin A: Activin RIIA[Biotinylated] Inhibitor Screening Assay Kit

The Activin A: Activin RIIA [Biotinylated] Inhibitor Screening Assay Kit is a chemiluminescence-based ELISA designed to measure the binding between human Activin A and Activin RIIA (receptor IIA), also known as ACVR2A, for screening and profiling applications. This kit comes with enough purified Activin A (amino acids 311-426) and biotinylated Activin Receptor IIA, streptavidin-HRP, assay buffer, and detection reagent for 100 enzyme reactions.Figure 1. Activin A: Activin RIIA[Biotinylated] Inhibitor Screening Assay Kit schematic.A 96-well plate is coated with Activin A. After blocking, biotinylated Activin RIIA (ACVR2A) is added in an optimized assay buffer. Next, unbound biotinylated Activin RIIA is washed away, and the plate is incubated with streptavidin-HRP. After a final wash, ELISA ECL substrate is added to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to the binding of Activin RIIA to Activin A.Need us to run inhibitor screens or profile your compounds against Activin A binding to Activin RIIA? Check out ourImmunotherapy Biochemical Screening Services.