Chemi-Verse™ PDGFRα (D842Y) Kinase Assay Kit

PDGFRα (platelet-derived growth factor receptor A), also known as CD140a, is one of the isoforms of the tyrosine kinase receptor for PDGF (platelet-derived growth factor), belonging to the family of RTK class III family of proteins. Binding of PDGF induces receptor dimerization and trans-phosphorylation, which leads to the recruitment of signaling and adaptor proteins and activation of downstream signaling cascades, such as the PI3K (phosphoinositide 3-kinase) -mTOR (mammalian target of rapamycin) . It has been implicated in the regulation of cell growth and survival, apoptosis, and differentiation. It has been identified as a potential target in eosinophilic leukemia cancer, inflammatory breast cancer, and gastrointestinal stromal tumors (GIST) . GIST is one of the most common mesenchymal cancers in the gastrointestinal tract, with 10-15% of the mutations leading to constitutively active PDGFRα. D842Y is a mutation in exon 18, and differently from other D842 mutations, D842Y is not imatinib resistant. The study of PDGFRα and the development of new inhibitors may benefit the cancer therapy field.

Catalog #ReagentAmountStorage100201PDGFRα (D842Y), GST-Tag*5 µg-80°C793345x Kinase Assay Buffer 11.5 ml-20°C79686500 µMATP50 µl-20°C40217Protein Tyrosine Kinase Substrate (Poly-Glu, Tyr 4:1) (10 mg/ml) 50 µl-20°C8254596-well plate, white1Room Temp*The concentration of the protein is lot-specific and will be indicated on the tube.