Chemi-Verse™ HCK Kinase Assay Kit

The Chemi-Verse™ HCK Kinase Assay Kit is designed to measure human HCK (hematopoietic cell kinase) tyrosine kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified HCK (amino acids 230-497), kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.Need us to run inhibitor screens or profile your compounds against HCK? Check out ourKinase Screening Services.

Product Specifications

Background

HCK (hematopoietic cell kinase) is a member of the Src non-receptor tyrosine kinase (NRTKs) family of proteins, mainly found in myeloid cells. It is localized in the cytoplasm of cells in a phosphorylated inactive form. Activation of cytokine receptors leads to phosphatase-induced dephosphorylation of HCK, resulting in its stimulation. It phosphorylates downstream partners, including PI3K (phosphoinositide 3-kinase) /AKT and STAT5 (signal transduced and activator of transcription 5) . It is involved in inflammation, phagocytosis, membrane remodeling and cell migration. It is also linked to cancer, being part of the CXCL12 (C-X-C motif chemokine 12) /CXCR4 (C-X-C chemokine receptor type 4) signaling in AML (acute myeloid leukemia) . It can bind to NLRP3 and induce caspase-1 activationin vitro. The excessive activation of the NLRP3 (NOD-, LRR- and pyrin domain containing protein 3) inflammasome is involved in diseases such as type 2 diabetes, atherosclerosis, MS (multiple sclerosis), Parkinson’s, and Alzheimer’s disease. The use of HCK inhibitors is promising in the treatment of inflammasome-related disorders and cancer.

UniProt

P08631

Applications

Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.

Format

Catalog #NameAmountStorage40440HCK, GST-Tag*2.5 µg-80°C793345x Kinase Buffer 11.5 ml-20°C79686500 µM ATP50 µl-20°C40217PTK Substrate (Poly-Glu, Tyr 4:1) (10 mg/ml) 50 µl-20°C82545White 96-well plate1Room Temp*The concentration of the protein is lot-specific and will be indicated on the tube.

Shipping Conditions

-80°C

Storage Conditions

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed

Notes

Troubleshooting GuideVisitbpsbioscience.com/assay-kits-faqfor detailed troubleshooting instructions. For all further questions, please email[email protected]