Chemi-Verse™ RSK4 Kinase Assay Kit

RSK4 (ribosomal S6 kinase 4) is a member of the serine/threonine RSK family of proteins. In vertebrates this family has four isoforms, RSK1-4, involved in cell growth, proliferation, motility, and apoptosis, acting as downstream effectors of the Ras/ERK (extracellular signal regulated kinase) pathway. RSK4 is found at high levels in the kidney, the brain, and the thyroid gland. It is linked to ESCC (esophageal squamous cell carcinoma) where it is found at high levels and phosphorylates GSK-3β to activate the β-catenin signaling pathway and leads to resistance to radiotherapy. Inhibition of this protein with the inhibitor BI-D1870 was able to increase the sensitivity of the ESCC cells to radiotherapy in animal models. In glioblastoma RSK4 phosphorylates EZH2 (enhancer of zeste homolog 2) and contributes to resistance to treatment with EZH2 inhibitors. RSK4 contributes to treatment resistance and cancer progression in kidney carcinoma, melanoma and RCC (renal cell carcinoma) cell lines, but the role of RSK4 as a tumor suppressor or oncogene may be cancer dependent and remains controversial. A deeper understanding of the role of this protein in cancer combined with the use of inhibitors alone or in combinatory therapy may prove a new therapeutic avenue in cancer therapy.

Catalog #NameAmountStorageRSK4*2 µg-80°C793345x Kinase Buffer 11.5 ml-20°C79686500 µM ATP50 µl-20°CRSK Substrate (5 mg/ml) 100 µl-20°C79696White 96-well plate1Room Temp*The concentration of the protein is lot-specific and will be indicated on the tube.

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