Set8 Chemiluminescent Assay Kit

The Set8 Chemiluminescent Assay Kit is an ELISA (enzyme-linked immunosorbent assay) -based assay designed to measure the histone methylase activity of Set8 (SET domain containing lysine methyltransferase 8) for screening and profiling applications. The Set8 assay kit comes in a convenient 96-well format, with enough recombinant purified Set8 enzyme (amino acids 195-252), substrate, antibodies, and all the reagents necessary for detection.Need us to run inhibitor screens or profile your compounds against Set8? Check out ourMethyltransferase Screening Services.

Product Specifications

Background

SET8 (PR/SET domain containing protein 8), also known as KMT5A (lysine-specific methylates 5A), belongs to the family of proteins that contain an SET domain. It has the specificity of being the only protein that monomethylates H4K20me1, but also participates in monomethylating proteins like p53 and PCNA (proliferating cell nuclear antigen) . It is involved in cell cycle regulation and DNA replication and repair. Abnormal expression of SET8 can lead to cancer, and this protein is found at high levels in several cancers, such as breast cancer. Inhibition of SET8 was found to increase cancer cell sensitivity to radiotherapy. The use of inhibitors alone or in combinatory therapy promises to bring benefits in cancer therapy.

UniProt

Q9NQR1

Applications

Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.

Format

Catalog #NameAmountStorage51008Set8, Active, GST-Tag*90 µg-80°C52120400 µMS-adenosylmethionine4 x 250 µl-80°C521904x HMT Buffer 43 ml-20°CPrimary Antibody 2312.5 µl-80°C52131HSecondary HRP-Labeled Antibody 210 µl-80°C52100Blocking Buffer 450 ml+4°C0.5M DTT2 x 200 µl-20°C79670ELISA ECL Substrate A (translucent bottle) 6 ml+4°CELISA ECL Substrate B (brown bottle) 6 ml+4°CWhite plate (or strips) pre-coated with Histone-Substrate1 plateRoom Temp*The concentration of the protein is lot-specific and will be indicated on the tube.

Shipping Conditions

-80°C

Storage Conditions

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed

Notes

Troubleshooting GuideVisitbpsbioscience.com/assay-kits-faqfor detailed troubleshooting instructions. For all further questions, please email[email protected]

Contraindications

The Set8 Chemiluminescent Assay Kit is compatible with up to 1% final DMSO concentration. Avoid the use of strong acids and bases, ionic detergents and high salt concentrations.