Chemi-Verse™ CDK9/CyclinK Kinase Assay Kit

The Chemi-Verse™ CDK9/CyclinK Kinase Assay Kit is designed to measure CDK9 (cyclin dependent kinase 9) /Cyclin K kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant CDK9/CyclinK complex, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.Need us to run inhibitor screens or profile your compounds against this kinase? Check out ourKinase Screening and Profiling Services.

Product Specifications

Background

Cyclin-dependent kinase 9 (CDK9) is the catalytic subunit of the positive transcription elongation factor b (P-TEFb) complex, which phosphorylates the C-terminal domain of RNA polymerase II, a key player in the production of mature RNA. P-TEFb also includes cyclinT or cyclinK. CDK9 translocates to the nucleus once it gets autophosphorylated, where it can associate with cyclinT or K. Hyperactivation of cyclins is known to result in cancer and the development of resistance to therapy, and CDK9-cyclinK is involved in genome integrity. Lack of CDK9 or cyclinK was shown to impair cell recovery after cells were stressed and potentiated DNA damage. Further understanding of the role of CKD9/cyclinK in health and disease and how to modulate it may prove beneficial for fields such as cancer therapy.

UniProt

CDK9: P50750; CyclinK: O75909

Applications

Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.

Format

Catalog #NameAmountStorage102119CDK9/CyclinK, GST-Tag*20 µg-80°C793345x Kinase Buffer 11.5 ml-20°C79686500 µM ATP50 µl-20°CPDKtide (1 mg/ml) 500 µl-20°C79696White 96-well plate1Room Temp*The concentration of the protein is lot-specific and will be indicated on the tube.

Shipping Conditions

-80°C

Storage Conditions

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Notes

Troubleshooting GuideVisitbpsbioscience.com/assay-kits-faqfor detailed troubleshooting instructions. For all further questions, please email[email protected]