Chemi-Verse™ TTBK2 Kinase Assay Kit

TTBK2, or tau tubulin kinase 2, belongs to the serine-threonine kinase family, involved in primary cilium assembly. Mutations in TTBK2 are responsible for spinocerebellar ataxia 11 (SCA11), which is characterized by loss of Purkinje cells and connection to other neurons in the cerebellum. Loss of TTBK2 in a conditional knockout identified the critical role of this protein in Purkinje cells, where lack of cilia resulted in abnormal intracellular calcium levels and in loss of VGLUT2 positive synapses from dendrites. Studies into the mechanism of action of TTBK2 have identified that mutant protein can interfere with peroxisome numbers and localization, impairing the traffic of SMO (smoothened) to the cilia. Further studies are required to identify the exact functions of this protein and how best to target it when impaired.

Catalog #NameAmountStorageTTBK2*1.25 µg-80°C793345x Kinase Assay Buffer 11.5 ml-20°C79686500 µM ATP50 µl-20°C78514Myelin basic protein (MBP), 5 mg/ml100 µl-20°C79696White 96-well plate1Room Temp*The concentration of the protein is lot-specific and will be indicated on the tube.

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