Chemi-Verse™ PAK3 Kinase Assay Kit

PAK3, also known as P21 protein activated kinase 3, beta-PAK, belongs to the group I of the PAK family of serine/threonine kinases. It is mostly found in neuronal cells, and it is involved in synapse formation and plasticity. Mutations in this protein can result in nonsyndromic X-linked mental retardation, and overexpression is found in neuroendocrine tumors. It has also been found at high levels in hepatocellular carcinoma (HCC), where it correlates with the stage of the disease, and in lung cancer. In non-small cell lung cancer (NSCLC), PAK3 levels seem to relate to Smad4 deficiency, as lack of Smad4 (mother against decapentaplegic homolog 4) reduces the expression of miR-495 and miR-543, which are inhibitors of PAK3 expression. PAK3 in turn activates the PAK3-JNK (c-Jun N-terminal kinase) -Jun pathway, which increases metastasis. Inhibition of PAK3 can thus be a promising tool in cancer therapy.

Catalog #NameAmountStoragePAK3*1 µg-80°C793345x Kinase Buffer 11.5 ml-20°C79686500 µM ATP50 µl-20°CPAKtide, 1 mg/ml500 µl-20°C79696White 96-well plate1Room Temp*The concentration of the protein is lot-specific and will be indicated on the tube.

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