Chemi-Verse™ CLK2 Kinase Assay Kit

The Chemi-Verse™ CLK2 Kinase Assay Kit is designed to measure CLK2 (CDC like kinase 2) kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant CLK2 kinase (amino acids 137-end), kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.Need us to run inhibitor screens or profile your compounds against this kinase? Check out ourKinase Screening and Profiling Services.

Product Specifications

Background

CDC-like kinase 2 (CLK2) is part of the dual-specificity protein kinase (DSK) family that phosphorylate serine/arginine rich (SR) proteins that are part of the spliceosomal complex and also regulate non-splicing proteins. It is involved in cell cycle progression, apoptosis, the DNA replication checkpoint, and regulation of telomere length. Dysfunction in pathways regulated by CLK2 can result in inflammatory and neurodegenerative diseases such as Alzheimer’s disease, in hyperglycemia, and in cancer. CLK2 is upregulated in breast cancer, non-small cell lung cancer (NSCLC), glioblastoma, and GSC (glioma stem-like cell) . CLK2 also contributes to fatty acid oxidation and ketogenesis and is involved in fatty liver disease. Recent findings demonstrated that CLK kinases activate PTP-1B (protein-tyrosine phosphatase) family members, and this phosphatase may be an important cellular target of CLK. The use of Lorecivivint, a CLK2 inhibitor, in a clinical trial for osteoarthritis was found to be effective and safe. The development of CLK2 inhibitors and a deeper understanding of its role in the multiple pathways where it is involved will prove crucial for the treatment of CLK2-linked diseases.

UniProt

P49760

Applications

Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.

Format

Catalog #NameAmountStorage40197CLK2, GST-Tag*2.5 µg-80°C793345x Kinase Buffer 11.5 ml-20°C79686500 µM ATP50 µl-20°C78514Myelin Basic Protein (MBP), 5 mg/ml50 µl-20°C79696White 96-well plate1Room Temp*The concentration of the protein is lot-specific and will be indicated on the tube.

Shipping Conditions

-80°C

Storage Conditions

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Notes

Troubleshooting GuideVisitbpsbioscience.com/assay-kits-faqfor detailed troubleshooting instructions. For all further questions, please email[email protected]