Chemi-Verse™ MAPKAPK2 Kinase Assay Kit

MAPKAPK2, or MAP kinase-activated protein kinase 2, is a serine/threonine kinase protein. MAPKAPK2 is phosphorylated by p38 MAP kinase (p38MAPK) and participates in multiple crucial cellular pathways: stress response, cell cycle, cell apoptosis and inflammatory responses. One of its main substrates is HSP27 (heat shoch protein 27), a protein involved in oxidative stress responses, but can also activate and inactivate RNA-binding proteins (RBPs) . By acting on RBPs it can regulate the expression of proto-oncogenes, cytokines, chemokines, and pro-inflammatory factors. It has been implicated in colorectal, skin, bladder and prostate cancer, and inflammatory diseases. The use of p38MAPK inhibitors can lead to hepatic and cardiac toxicology and central nervous disorders likely because p38MAPK can interact with more than 60 partners involved in multiple pathways. MAPKAPK2 can be seen as a cell-cycle checkpoint and has become an attractive target for combination-based therapies in cancer. It has also been considered a disease-modifying anti-rheumatic drug (DMARD) ligand, and inhibitors of MAPKAPK2 may prove beneficial for the treatment of inflammatory diseases such as rheumatoid arthritis.

Catalog #NameAmountStorage40116MAPKAPK2, His-Tag*>1 µg-80°C793345x Kinase Buffer 11.5 ml-20°C79686500 µM ATP50 µl-20°CHSP27tide (5 mg/ml) 100 µl-20°C79696White 96-well plate1Room Temp*The concentration of the protein is lot-specific and will be indicated on the tube.

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