Chemi-Verse™ PI3 Kinase (p110α/p85α) Kinase Assay Kit

PI3 (phosphoinositide 3) kinases, or phosphatidylinositol 3 kinases, are a family of proteins that can be subdivided into four classes: I, II, III and IV. Class I are involved in converting PI (4,5) P2 (phosphatidylinositol (4,5) -biphosphate) into PI (3,4, 5) P3 (phosphatidylinositol (3,4, 5) -triphosphate) when activated by tyrosine kinase receptors and G-protein coupled receptors. They are heterodimeric proteins with a regulatory and a catalytic subunit. The heterodimer between p110 (catalytic subunit) and p85 (regulatory subunit) belongs to class IA. p110 and p85 have three variants each. p110α is ubiquitously expressed, and p85α is the most abundant variant of p85. Class I PI3K participates in cell signaling, mostly via the activation of PKB (protein kinase B) and the PI3K/AKT/mTOR pathway. Dysfunction of these kinases impacts cell growth and differentiation, and mutations in p110α have been linked to cancer. At least three isoform-specific inhibitors are approved by FDA for the treatment of lymphoma and leukemia. Further studies will help identify more selective inhibitors with a good tolerance that can bypass the development of drug resistance.

Catalog #NameAmountStorage40621PI3 kinase (p110α/p85α) *2.5 µg-80°C793345x Kinase Buffer 11.5 ml-20°C79686500 µM ATP50 µl-20°C40560PI3K Lipid Substrate (Packaged separately, Do Not Freeze!) 500 µl4°C79696White 96-well plate1Room Temp*The concentration of the protein is lot-specific and will be indicated on the tube.

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The final concentration of DMSO in the assay should not exceed 1%.ThePI3K lipid substrate is shipped separately on ice. Please store it at 4°C upon arrival (DO NOT FREEZE the PI3K lipid substrate) .