Chemi-Verse™ SIK1 Kinase Assay Kit

The Chemi-Verse™ SIK1 Kinase Assay Kit is designed to measure SIK1 (salt-inducible kinase 1) kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant SIK1 kinase, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.Need us to run inhibitor screens or profile your compounds against this kinase? Check out ourKinase Screening and Profiling Services.

Product Specifications

Background

SIK (salt-inducible kinases) are serine/threonine kinases of the AMPK (AMP-activated protein kinase) family. They are involved in gluconeogenesis and lipid metabolism inhibition. Dysfunction of SIK has been linked to cancer, due to its association with the TGFβ (transforming growth factor β) -Smad, AKT (protein kinase B), Hippo and NF-κB (nuclear factor kappa-light chain enhancer of activated B cells) pathways. The role of SIK proteins in cancer is complex, with some family members acting as oncogenes while others functioning as suppressors. SIK1 is present at high levels in the adrenal cortex, adipose and neural tissues. SIK1 acts as tumor suppressor in ovarian, lung, colorectal and breast cancer, pancreatic and hepatocellular carcinoma. It inhibits the epithelial-mesenchymal transition (EMT) by downregulating transcription factors involved in inhibition of E-cadherin expression, such as ZEB1 (zinc-finger E-box binding homeobox 1) and ZEB2. MicroRNAs, such as miR-141 and miR17, target SIK1 and promote cancer progression. The development of SIK inhibitors is the focus of intense research, but further studies are needed to identify isoform-specific inhibitors that can be used in cancer therapy.

UniProt

P57059

Applications

Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.

Format

Catalog #NameAmountStorageSIK11.25 µg-80°C793345x Kinase Buffer 11.5 ml-20°C79686500 µM ATP50 µl-20°CAmara Peptide (1 mg/ml) 500 µl-20°C79696White 96-well plate1Room Temp*The concentration of the protein is lot-specific and will be indicated on the tube.

Shipping Conditions

-80°C

Storage Conditions

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Notes

Troubleshooting GuideVisitbpsbioscience.com/assay-kits-faqfor detailed troubleshooting instructions. For all further questions, please email[email protected]