Chemi-Verse™ Kinase Aurora C Assay Kit

The Chemi-Verse™ Aurora Kinase C Assay Kit is a luminescence assay designed to measure Aurora Kinase C activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant Aurora Kinase C, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.Need us to run inhibitor screens or profile your compounds against this kinase? Check out ourKinase Screening and Profiling Services.

Product Specifications

Background

Aurora Kinase C, also known as serine/threonine-protein kinase 13, is part of the Aurora subfamily. Aurora Kinase C is a CPC (chromosomal passenger complex) protein and forms a complex with Aurora Kinase B and centromere proteins. It plays a role in microtubule organization during mitosis and meiosis, mostly in meiosis. It has a pattern of expression and subcellular localization that varies with the cell cycle stage, in agreement with its role in cell cycle regulation. It has oncogenic activity and is overexpressed in cancer cell lines, by performing similar functions to Aurora Kinase B and other still unknown mechanisms. Mutations that inactivate this protein result in male infertility, being the main cause of infertility in North African men. The use of inhibitors can increase the effectiveness of chemotherapy and has been shown to stop the progress and growth of several cancers. Further studies will elucidate the exact mode of action of Aurora Kinase C in cancer development and progression and open new therapeutic avenues.

UniProt

Q9UQB9

Applications

Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.

Format

Catalog #NameAmountStorage40178Aurora Kinase C, GST-Tag Recombinant *5 µg-80°C793345x Kinase Buffer 11.5 ml-20°C79686500 µM ATP50 µl-20°C78514Myelin basic protein (MBP), 5 mg/ml50 µl-20°C79696White 96-well plate1Room Temp*The concentration of the protein is lot-specific and will be indicated on the tube.

Shipping Conditions

-80°C

Storage Conditions

This assay kit will perform optimally for up to6 months from date of receipt when the materials are stored as directed.

Notes

Troubleshooting GuideVisitbpsbioscience.com/assay-kits-faqfor detailed troubleshooting instructions. For all further questions, please email[email protected]