Chemi-Verse™ Aurora Kinase B Assay Kit

The Chemi-Verse™ Aurora Kinase B Assay Kit is a luminescence assay designed to measure Aurora Kinase B kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant Aurora Kinase B, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.Need us to run inhibitor screens or profile your compounds against this kinase? Check out ourKinase Screening and Profiling Services.

Product Specifications

Background

Aurora Kinase B, also known as serine/threonine-protein kinase 5, is part of the Aurora subfamily. Aurora Kinase B is a CPC (chromosomal passenger complex) protein. It plays a role in microtubule organization during mitosis and meiosis, forming a complex with Survinin, Borealin and INCENP (inner centromere protein) and binding to EB1 (end-binding protein 1) . It has a pattern of expression and subcellular localization that varies with the cell cycle stage, in agreement with its role as cell cycle regulator. It has oncogenic activity, being able to lead to the development of cells with abnormal number of chromosomes. Aurora Kinase B also promotes cancer by decreasing expression of p21, hyperactivating CDK1 (cyclin-dependent kinase 1) and regulating class IIa HDACs (histone deacetylase) . Interestingly, the use of Aurora inhibitors can result in an increase in the number of chromosome alterations in cancer cells, resulting in cell death, and can also decrease expression of cyclin B1 and D1 and elevate caspase 3 levels in lymphoma cells. Further studies will elucidate the exact mode of action of Aurora Kinase B in cancer development and progression and open new therapeutic avenues.

UniProt

Q96GD4

Applications

Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.

Format

Catalog #NameAmountStorage40002Aurora Kinase B GST-Tag, His-Tag*20 µg-80°C793345x Kinase Buffer 11.5 ml-20°C79686500 µM ATP50 µl-20°C78514Myelin basic protein (MBP), 5 mg/ml50 µl-20°C79696White 96-well plate1Room Temp*The concentration of the protein is lot-specific and will be indicated on the tube.

Shipping Conditions

-80°C

Storage Conditions

This assay kit will perform optimally for up to6 months from date of receipt when the materials are stored as directed

Notes

Troubleshooting GuideVisitbpsbioscience.com/assay-kits-faqfor detailed troubleshooting instructions. For all further questions, please email[email protected]