Chemi-Verse™ CDK18/Cyclin Y Kinase Assay Kit

The Chemi-Verse™ CDK18/Cyclin Y Kinase Assay is a luminescent assay designed to measure CDK18 (cyclin-dependent kinase 18) /Cyclin Y kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant CDK18/Cyclin Y complex, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.Need us to run inhibitor screens or profile your compounds against this kinase? Check out ourKinase Screening and Profiling Services.

Product Specifications

Background

CDK18 (cyclin dependent kinase 18) is a member of the cyclin-dependent protein kinase family involved in cell cycle, transcription and splicing. CDK associates with cyclins, which induce a conformational change that results in a dramatic increase of kinase activity. Cyclin levels vary during the cell cycle, allowing cyclins to regulate CDK activity in the cell. Dissociation of the complex returns CDK to its basal activity, and CDK is degraded by ubiquitin mediated proteolysis. Lack of regulation in cell cycle can result in apoptosis and finally in cancer, neurodegenerative diseases (Alzheimer’s or Parkinson disease) and cardiovascular complications (stroke) . The understanding of the mechanisms involved in cell cycle regulation, and its control via the use of small molecule inhibitors will open new therapeutic avenues for the treatment of cancer and neurodegenerative diseases.

UniProt

CDK18: Q07002; Cyclin Y: Q8ND76

Applications

Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.

Format

Catalog #NameAmountStorage100608CDK18/Cyclin Y, GST-Tag Recombinant*10 µg-80°C793345x Kinase Assay Buffer 11.5 ml-20°C79686500 µM ATP50 µl-20°CRb Protein (2 mg/ml) 125 µl-20°C79696White 96-well plate1Room Temp*The concentration of the protein is lot-specific and will be indicated on the tube.

Shipping Conditions

-80°C

Storage Conditions

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Notes

Troubleshooting GuideVisitbpsbioscience.com/assay-kits-faqfor detailed troubleshooting instructions. For all further questions, please email[email protected]