Chemi-Verse™ CDK8/Cyclin C Kinase Assay Kit

The Chemi-Verse™ CDK8/Cyclin C Kinase Assay is a luminescent assay designed to measure CDK8 (cyclin-dependent kinase 8) /Cyclin C kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant complex of CDK8/Cyclin C, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.Need us to run inhibitor screens or profile your compounds against this kinase? Check out ourKinase Screening and Profiling Services.

Product Specifications

Background

CDK8 (cyclin dependent kinase 8) is a member of the cyclin-dependent protein kinase family involved in cell cycle regulation, transcription and splicing. CDK associates with cyclins, which induce a conformational change that results in a dramatic increase of the kinase activity. Cyclin levels vary during the cell cycle, which allows cyclins to regulate CDK activity in the cell. Dissociation of the complex returns CDK to its basal activity, and CDK is degraded by ubiquitin mediated proteolysis. In addition to cyclin, CDK8 is part of a Mediator complex that includes CDK19, MED12 and MED13 and acts as a negative regulator of RNA Polymerase II. CDK8 regulates several pathways, such as Notch, Wnt/β-catenin and Sonic Hedgehog (Shh) and has been described as an oncogenic driver and implicated in several cancer types (breast, ovarian, gastric cancer and leukemia) . The understanding of the mechanisms involved in cell cycle regulation, and its control via the use of small molecule inhibitors will open new therapeutic avenues for the treatment of cancer.

UniProt

CDK8: P49336: CyclinC: P24863

Applications

Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.

Format

Catalog #NameAmountStorageCDK8/Cyclin C, GST-Tag *15 µg-80°C793345x Kinase Assay Buffer 11.5 ml-20°C79686500 µM ATP50 µl-20°C79604CDK Substrate Peptide 2125 µl-20°C79696White 96-well plate1Room Temp*The concentration of the protein is lot-specific and will be indicated on the tube.

Shipping Conditions

-80°C

Storage Conditions

This assay kit will perform optimally for up to6 monthsfrom date of receipt when the materials are stored as directed.

Notes

Troubleshooting GuideVisitbpsbioscience.com/assay-kits-faqfor detailed troubleshooting instructions. For all further questions, please email[email protected]