NEK7 Kinase Assay Kit

The NEK7 Kinase Assay Kit is designed to measure NEK7 (NIMA-related Kinase 7) serine/threonine kinase activity for screening and profiling applications using ADP-Glo™as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant NEK7 kinase, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

Product Specifications

Background

The NIMA-related Kinase (NEK) family are serine/threonine kinases, also known as mitotic kinases. NEK7 is activated via phosphorylation at Ser-195, and is involved in microtubule nucleation, mitotic spindle formation and cytokinesis. Overexpression or abnormal activity of NEK7 can lead to uncontrolled cell division and growth, a hallmark of cancer. NEK7 is also involved in Muckle-Wells Syndrome and Cinca Syndrome. Protein kinase inhibitors have been extensively studied as potential therapeutical approaches for cancer, with attention starting to focus on NEK7 specific inhibitors as potential neoplastic drugs.

UniProt

Q8TDX7

Applications

Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.

Format

Catalog #NameAmountStorage40141NEK7, GST-Tag*40 µg-80°C79334Kinase Buffer 1 (5x) 1.5 ml-20°C79686500 µM ATP50 µl-20°C78514MBP (5 mg/ml) 50 µl-20°C79696White 96-well plate1Room Temp*The concentration of the protein is lot-specific and will be indicated on the tube.

Shipping Conditions

-80°C

Storage Conditions

This assay kit will perform optimally for up to6 monthsfrom date of receipt when the materials are stored as directed.

Notes

Troubleshooting GuideVisitbpsbioscience.com/assay-kits-faqfor detailed troubleshooting instructions. For further questions, please email[email protected]