FGFR4 Kinase Assay Kit

The Fibroblast growth factor receptor 4 (FGFR4) Kinase Assay Kit is designed to measure FGFR4 kinase activity for screening and profiling applications using ADP-Glo® as a detection reagent.

Product Specifications

Background

FGFR4 (also known as CD334) is a transmembrane receptor with tyrosine kinase activity. The extracellular portion of the receptor contains three immunoglobulin-like domains and is responsible for binding fibroblast growth factor 1 (FGF1) . It contains a single transmembrane domain followed by the cytosolic kinase domain. FGFR4 may be involved in ovarian cancer as well as adrenocortical carcinoma and is a promising therapeutic target in oncology applications.

UniProt

P22455

Applications

Study enzyme kinetics and screen small molecular inhibitors for drug discovery and high throughput (HTS) applications.

Format

Catalog #NameAmountStorage40213FGFR4*20 µg-80°C79334Kinase Assay Buffer 1 (5x) 1.5 ml-20°C79686ATP (500 µM) 50 µl-20°C40217PTK Substrate (Poly-Glu, Tyr 4:1) (10 mg/ml) 50 µl-20°C79696White 96-well plate1Room Temp*The concentration of the protein is lot-specific and will be indicated on the tube

Shipping Conditions

-80°C

Storage Conditions

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Notes

Troubleshooting GuideVisitbpsbioscience.com/assay-kits-faqfor detailed troubleshooting instructions. For all further questions, please email[email protected]