MCL-1 TR-FRET Assay Kit (Rat)

Induced myeloid leukemia cell differentiation protein (MCL-1) is part of the BCL-2 family of proteins that regulates apoptosis. The intrinsic (mitochondrial) apoptotic pathway is activated by intracellular signals strictly controlled by the BCL-2 family of proteins and are grouped into the antiapoptotic, activator, and sensitizer subsets. All function through complex interactions to regulate the integrity of the mitochondrial membrane. As part of the antiapoptotic subset, MCL-1 inhibits mitochondrial outer membrane permeabilization (MOMP) and the release of cytochrome C from mitochondria. MCL-1 is essential for the survival of various cell types that include the nervous system, cardiomyocytes and lymphocytes. Overexpression of MCL-1 is frequently observed in many tumor types and is closely associated with tumorigenesis and drug resistance. Because of its ability to strictly regulate cell fate, MCL-1 is a target of interest for many drug therapies.

Catalog #NameAmountStorage101299Rat MCL-1*5 µg-80°C79507MCL-1 Peptide Ligand400 reactions-80°C30017Anti-His Tb-labeled donor2 x 10 µl-20°CDye-labeled acceptor2 x 10 µl-20°C3x MCL TR-FRET Assay Buffer4 ml-20°C79969384-well white microplate1Room Temp*The initial concentration of Rat MCL-1 is lot-specific and will be indicated on the tube containing the protein.

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