BTK (C481S) Kinase Assay Kit

The BTK (C481S) Kinase Assay Kit is designed to measure BTK (C481S) kinase activity for screening and profiling applications using ADP-Glo® as a detection reagent.

Product Specifications

Background

Bruton’s tyrosine kinase (BTK), is an enzyme that plays a role in the functionality and maturation of B cells. The BTK pathway has implications for a number of autoimmune disorders including isolated growth hormone deficiency type III and rheumatoid arthritis. However, as therapies have progressed, specifically in chronic lymphocytic leukemia (CLL), resistance has become more common in patients where the disease has progressed due to prolonged therapy. A mutation in the BTK 481 cysteine residue to which inhibitors bind covalently is often linked to BTK inhibition (BTKi) in CLL patients where resistance has been led to disease progression. Targeting the BTK mutation of interest (C481S) has shown promising clinical efficacy in treating CLL patients that are no longer responding to common therapy.

UniProt

Q06187

Applications

Study enzyme kinetics and screen small molecular inhibitors for drug discovery and high throughput (HTS) applications.

Format

Catalog #NameAmountStorage101039BTK (C481S), His-Tag*10 µg-80°C793345X Kinase Assay Buffer 11.5 ml-20°C79686ATP (500 µM) 100 µl-20°C40217PTK substrate Poly (Glu:Tyr: 4:1) (10 mg/ml) 100 µl-20°C79696White 96-well plate1Room Temp*The concentration of the protein is lot-specific and will be indicated on the tube

Shipping Conditions

-80°C

Storage Conditions

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Notes

Troubleshooting GuideVisitbpsbioscience.com/assay-kits-faqfor detailed troubleshooting instructions. For all further questions, please email[email protected]