CD137:CD137L[Biotinylated] Inhibitor Screening Assay Kit

The CD137:CD137L [Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of CD137:CD137L interaction. The assay takes advantage of the highly sensitive detection of biotin-labeled CD137L by streptavidin-HRP. First, CD137 is coated on a 96-well plate. Next, CD137L-biotin is incubated with CD137. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader.Need us to run inhibitor screens or profile your compounds against CD137:CD137L[Biotinylated]? Check out ourImmunotherapy Biochemical Screening Services.

Product Specifications

Background

CD137, also known as 4-1BB, is a prototype co-stimulatory molecule expressed on T cells. CD137 interaction with its cognate ligand, CD137L, also known as 4-1BBL, results in increased T cell activation and proliferation. Accumulating evidence shows a role for CD137:CD137L signaling in inflammation, suggesting that inhibition of this pathway may provide a therapeutic avenue to treat autoimmune and inflammatory diseases.

UniProt

CD137: Q07011; CD137L: P41273

Applications

Screen and profile inhibitors of CD137 binding to CD137L.

Format

Catalog #NameAmountStorage71170CD137 (4-1BB), Fc fusion*10 µg-80°C100239CD137L (4-1BB ligand), His, Avi, Biotin2 µg-80°C79742Streptavidin-HRP10 µl4°C793113x Immuno Buffer 150 ml-20°C79728Blocking Buffer 250 ml4°C79670ELISA ECL substrate A (transparent bottle) 6 mlRoom TempELISA ECL substrate B (brown bottle) 6 mlRoom Temp7969696-well plate, white1Room Temp*The concentration of the protein is lot-specific and will be indicated on the tube