E.coli Host Cell Protein ELISA Kit, G3 (6-Strain Combo-K-12, BL21, JM109, DH5α, MC1061, TOP10F)
ELISA Kit for the quantitative Measurement of E. coli HCP Residues in Protein Purification Process and End-Product
Product Specifications
Reproducibility
1) Intra Variation: 3.2 - 8 % || 2) Inter Variation: 4.5 - 10.5 %
Certification
ISO 13485:2016
Reactivity
E. coli (6-Strain Combo-K-12, BL21, JM109, DH5α, MC1061, TOP10F)
Cross Reactivity
No cross-reaction exists between the homologous antigens.
Target
E. coli HCP residue
Type
Sandwich ELISA
Applications
ELISA
Field of Research
ELISA Kit for the quantitative Measurement of host cell protein (HCP) residues in biopharmaceuticals by E. coli (6-Strain Combo-K-12, BL21, JM109, DH5α, MC1061, TOP10F) expression systems
Assay Type
Sandwich ELISA
Assay Performance Time
3.5 hours
Sample Type
Cell Culture Supernatants, Protein Purification Process, and End-Product
Detection Range
1.95-125ng/ml
Sensitivity
LOD: 0.122ng/ml; LOQ: 1.95ng/ml
Format
96 Tests
Components
1 Pre-Coated Microplate (Detachable) 2 Standard (Stock Solution) -500μg/mL 3 Detection antibody (100x) 4 TMB Substrates 5 Stop Solution 6 Wash Solution (100x) 7 Diluent Buffer (10x) 8 Plate Sealer 9 Instruction Manual
Shipping Conditions
Gel Packs
Storage Conditions
2-8°C
Inter CV
4.5-10.5 %
Intra CV
3.2-8 %
Species
E. coli
Documents
Frequently Asked Questions
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