Human Alpha-Synuclein Pre-formed Fibrils Protein, His Tag

Alpha-synuclein is a neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. It acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5. Abnormalities in alpha-synuclein are implicated in the pathogenesis of Parkinson's disease (PD) . Alpha-synuclein is present in Lewy-bodies, the neuropathological hallmark of PD, and the protein and its aggregation have been widely linked to neurotoxic pathways that ultimately lead to neurodegeneration.

Product Specifications

Background

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 16.3 kDa.

Accession Number

P37840-1

Expression Region

Met 1 - Ala 140

Host

E. coli

Target

Alpha-Synuclein

Conjugation

Unconjugated

Tag

C-10xHis

Applications

1. Sonication Conditions, Dissolution, Aliquoting, Storage, and Notes for PFFThawing:Aliquoting:Storage:Sonication:Note:2. Recommendations for PFF Use in Animal ModelsMinimize Freeze-Thaw Cycles:PFF Maintenance During Injections:

Stability

The product should be stored at -70°C or room temperature for short storage. Do not store fibrils on ice or at 4°C; The unsonicated fibril is validated to be stable after storage at -70°C for 1 year under sterile conditions; The sonicated fibril should be stored at -70°C for not more than 8 weeks.