Human beta-Glucuronidase/GUSB Protein, His Tag (active enzyme)
Human beta-Glucuronidase Protein, His Tag (BEB-H52H3) is expressed from human 293 cells (HEK293) . It contains AA Leu 23 - Thr 651 (Accession # P08236) .
Product Specifications
Background
Human beta -Glucuronidase (EC 3.2.1.31) encoded by the GUSB gene is a lysosomal hydrolase involved in the stepwise degradation of glucuronic acid-containing glycosaminoglycans that include heparan sulfate, chondroitin sulfate and hyaluronan. The enzyme is only active on the glucuronic acid of the non-reducing end. The native protein has been reported as a tetrameric glycoprotein composed of identical subunits.
Specifications
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 74.5 kDa. The protein migrates as 75-85 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Accession Number
P08236
Expression Region
Leu 23 - Thr 651
Host
HEK293
Target
Beta-Glucuronidase/GUSB
Conjugation
Unconjugated
Tag
C-10xHis
Source
Human
Stability
● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin
1.0 EU per μg
Purity
95%
Format
Powder
Buffer
50 mM Tris, 150 mM NaCI, pH7.5
Additives
Trehalose
Molecular Weight
74.5 kDa
Shipping Conditions
RT
Storage Conditions
-20°C
Package Size
500ug*2
Product Datasheet
Https://www.acrobiosystems.com/static/main/products/pdf/ds/BEB-H52H3.pdf
Species
Human
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