Anti-SAP30L Antibody

Rabbit Polyclonal Antibody specific to SAP30L

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

HCV non-structural protein 4A-transactivated protein 2, Sin3 corepressor complex subunit SAP30L, Sin3-associated protein p30-like

Gene Name

SAP30L

NCBI Gene ID

UniProt

Q9HAJ7

Cellular Locus

[Isoform 1]: Nucleus, nucleolus

Host

Rabbit

Reactivity

Human

Immunogen

Synthetic peptide corresponding to amino acids 165-183 (VKSNKSRLDQKSEGGKQLE) of human SAP30L conjugated to KLH at the N- terminus.

Target Antigen

Histone deacetylase complex subunit SAP30L

Target

SAP30L

Clonality

Polyclonal

Type

Antibody

Applications

WB, ELISA

Field of Research

Neuroscience

Purification Method

Purified by immunoaffinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

[Isoform 1]: Functions as transcription repressor, probably via its interaction with histone deacetylase complexes (PubMed:16820529, PubMed:18070604). Involved in the functional recruitment of the class 1 Sin3-histone deacetylase complex (HDAC) to the nucleolus (PubMed:16820529). Binds DNA, apparently without sequence-specificity, and bends bound double-stranded DNA (PubMed:19015240). Binds phosphoinositol phosphates (phosphoinositol 3-phosphate, phosphoinositol 4-phosphate and phosphoinositol 5-phosphate) via the same basic sequence motif that mediates DNA binding and nuclear import (PubMed:19015240, PubMed:26609676). {PubMed:16820529, PubMed:18070604, PubMed:19015240, PubMed:26609676}.; [Isoform 2]: Functions as transcription repressor; isoform 2 has lower transcription repressor activity than isoform 1 and isoform 3. {PubMed:18070604}.; [Isoform 3]: Functions as transcription repressor; its activity is marginally lower than that of isoform 1. {PubMed:18070604}.

Additionnal Information

Immunoblotting: use at 1:500 dilution. Bands of approximately 21-22 kDa (endogenous) and/or 30 kDa (wild-type transfected) are detected. <br><br>Positive control: MCF7 or HEK293 cell lysate.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.

Specificity

This antibody detects wild-type transfected, endogenous, or GST- SAP30L fusion protein in human cell lines.

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

A novel TGF-beta up-regulated mRNA species, the Sin3-associated protein 30- like, SAP30L has been identified. The predicted nuclear localization signal of SAP30L is sufficient for nuclear transport of the protein although mutating it does not completely remove SAP30L from the nuclei. By reason of its nuclear localization and close homology to SAP30, it is thought that SAP30L might have a role in recruiting the Sin3-histone deacetylase complex to specific co- repressor complexes in response to TGF- beta, leading to the silencing of proliferation-driving genes in the differentiating intestinal epithelial cells.