SingleFlowEx CD3 APC-Cy™7

SingleFlowEx is fluorochrome-labeled monoclonal antibody single reagent for use in laboratory-developed multicolor flow cytometry tests in order to examine human clinical samples. SingleFlowEx can be used in various multicolor combinations. Laboratory shall establish the SingleFlowEx performance characteristics in conjunction with other reagents in normal and pathological samples.

Product Specifications

Certification

RUO (GMP)

Clone

SK7

Applications

FC (QC tested)

Concentration

0.025 mg/mL

Format

APC-Cy™7

Buffer

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Qiao D, Li L, Guo J, Lao S, Zhang X, Zhang J, Wu C: Mycobacterium tuberculosis culture filtrate protein 10-specific effector/memory CD4⁺ and CD8⁺ T cells in tubercular pleural fluid, with biased usage of T cell receptor Vβ chains. Infect Immun. 2011 Aug;79 (8) :3358-65., URL: https://pubmed.ncbi.nlm.nih.gov/21606188/, *Wood GS, Burns BF, Dorfman RF, Warnke RA: The immunohistology of non-T cells in the acquired immunodeficiency syndrome. Am J Pathol. 1985 Sep;120 (3) :371-9., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=am+j+pathol+120+371, *Li B, Wang H, Dai J, Ji J, Qian W, Zhang D, Hou S, Guo Y: Construction and characterization of a humanized anti-human CD3 monoclonal antibody 12F6 with effective immunoregulation functions. Immunology. 2005 Dec;116 (4) :487-98., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=li+b+wang+h+dai+j+ji+j , *Recio MJ, Moreno-Pelayo MA, Kiliç SS, Guardo AC, Sanal O, Allende LM, Pérez-Flores V, Mencía A, Modamio-Hø ybjø r S, Seoane E, Regueiro JR: Differential biological role of CD3 chains revealed by human immunodeficiencies. J Immunol. 2007 Feb 15;178 (4) :2556-64., URL: http://www.ncbi.nlm.nih.gov/pubmed/17277165 , *Guttman-Yassky E, Vugmeyster Y, Lowes MA, Chamian F, Kikuchi T, Kagen M, Gilleaudeau P, Lee E, Hunte B, Howell K, Dummer W, Bodary SC, Krueger JG: Blockade of CD11a by efalizumab in psoriasis patients induces a unique state of T-cell hyporesponsiveness. J Invest Dermatol. 2008 May;128 (5) :1182-91., URL: http://www.ncbi.nlm.nih.gov/pubmed/18239614 , *Fromm JR, Thomas A, Wood BL: Characterization and purification of neoplastic cells of nodular lymphocyte predominant hodgkin lymphoma from lymph nodes by flow cytometry and flow cytometric cell sorting. Am J Pathol. 2017 Feb;187 (2) :304-317., URL: https://pubmed.ncbi.nlm.nih.gov/27998726/, *van Dongen JJ, Krissansen GW, Wolvers-Tettero IL, Comans-Bitter WM, Adriaansen HJ, Hooijkaas H, van Wering ER, Terhorst C: Cytoplasmic expression of the CD3 antigen as a diagnostic marker for immature T-cell malignancies. Blood 1988 Mar;71 (3) :603-12., URL: https://pubmed.ncbi.nlm.nih.gov/3278747/, *Kužílková D, Puñet-Ortiz J, Aui PM, Fernández J, Fišer K, Engel P, van Zelm MC, Kalina T: Standardization of workflow and flow cytometry panels for quantitative expression profiling of surface antigens on blood leukocyte subsets: An HCDM CDMaps initiative. Front Immunol. 2022 Feb 11:13:827898. , URL: https://pubmed.ncbi.nlm.nih.gov/35222411/, *Use of this product has not been published., URL:

Other References

*Huang Y, Wange RL: T cell receptor signaling: beyond complex complexes. J Biol Chem. 2004 Jul 9;279 (28) :28827-30., URL: https://pubmed.ncbi.nlm.nih.gov/15084594/, *Alarcón B, Swamy M, van Santen HM, Schamel WW: T-cell antigen-receptor stoichiometry: pre-clustering for sensitivity. EMBO Rep. 2006 May;7 (5) :490-5., URL: https://pubmed.ncbi.nlm.nih.gov/16670682/, *Kuhns MS, Davis MM, Garcia KC: Deconstructing the form and function of the TCR/CD3 complex. Immunity. 2006 Feb;24 (2) :133-9., URL: https://pubmed.ncbi.nlm.nih.gov/16473826/