Cyanine5 alkyne

Cyanine5 alkyne (Alkyne-Cy5) is a fluorescent dye used to label azide proteins and can be used to analyse post-translational modifications of proteins, glycosylation etc. Cyanine5 alkyne can also be used as a mitochondrial OXPHOS inhibitor to inhibit the growth of cancer stem cells (CSC) [1][2]. Cyanine5 alkyne is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

Product Specifications

CAS Number

[1223357-57-0]

Product Name Alternative

Alkyne-Cy5

UNSPSC

12171500

Target

Mitochondrial Metabolism; Oxidative Phosphorylation

Type

Dye Reagents

Related Pathways

Metabolic Enzyme/Protease

Applications

Cancer-Kinase/protease

Field of Research

Cancer

Assay Protocol

https://www.medchemexpress.com/cyanine5-alkyne.html

Purity

98.15

Solubility

DMSO : 125 mg/mL (ultrasonic)

Smiles

C#CCNC(CCCCC[N+]1=C(/C=C/C=C/C=C2N(C)C3=C(C=CC=C3)C\2(C)C)C(C)(C)C4=C1C=CC=C4)=O.[Cl-]

Molecular Formula

C35H42ClN3O

Molecular Weight

556.18

References & Citations

[1]Amanda R Burnham-Marusich, et al. Size-matched alkyne-conjugated cyanine fluorophores to identify differences in protein glycosylation. Electrophoresis. 2014 Sep;35 (18) :2621-5.|[2]Camillo Sargiacomo, et al. MitoTracker Deep Red (MTDR) Is a Metabolic Inhibitor for Targeting Mitochondria and Eradicating Cancer Stem Cells (CSCs), With Anti-Tumor and Anti-Metastatic Activity In Vivo. Front Oncol. 2021 Jul 30;11:678343.