Phospho PKC θ (Y90) rabbit pAb

Catalytic activity: ATP + a protein = ADP + a phosphoprotein. Cofactor: Magnesium. Domain: The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain. enzyme regulation: Three specific sites; Thr-538 (activation loop of the kinase domain), Ser-676 (turn motif) and Ser-695 (hydrophobic region), need to be phosphorylated for its full activation. function: PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. function: This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production. PTM: Autophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PKC upon antigen receptor ligation. similarity: Belongs to the protein kinase superfamily. similarity: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. similarity: Contains 1 AGC-kinase C-terminal domain. similarity: Contains 1 C2 domain. similarity: Contains 1 protein kinase domain. similarity: Contains 2 phorbol-ester/DAG-type zinc fingers. subunit: Interacts with TXNL2/PICOT. tissue specificity: Skeletal muscle, megakaryoblastic cells and platelets.