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Anti-PSE1/Ku Protein Antibody

Mouse Monoclonal Antibody specific to PSE1/Ku Protein

Product Specifications

Product Name Alternative

EC 3.6.4.-, EC 4.2.99.-, 5'-deoxyribose-5-phosphate lyase Ku70, 5'-dRP/AP lyase Ku70, ATP-dependent DNA helicase 2 subunit 1, ATP-dependent DNA helicase II 70 kDa subunit, CTC box-binding factor 75 kDa subunit, CTC75, CTCBF, DNA repair protein XRCC6, Ku autoantigen protein p70 homolog, Ku70

Gene Name

Xrcc6

NCBI Gene ID

<a href="https://www.ncbi.nlm.nih.gov/gene/?term=Xrcc6">Xrcc6</a>

UniProt

P23475

Cellular Locus

Nucleus, Chromosome

Host

Mouse

Reactivity

Human

Immunogen

Proximal sequence element (PSE1) of the human U1 small nuclear RNA extracted from human placenta.

Target Antigen

X-ray repair cross-complementing protein 6

Target

PSE1/Ku Protein

Clonality

Monoclonal

Isotype

IgG2b

Type

Antibody

Applications

WB, Gel Shift

Field of Research

Protein Transcription

Purification Method

Purified by Protein G affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Single-stranded DNA-dependent ATP-dependent helicase that plays a key role in DNA non-homologous end joining (NHEJ) by recruiting DNA-PK to DNA (By similarity). Required for double-strand break repair and V(D)J rUniProtKB:P12956, PubMed:12145306}.

Additionnal Information

These antibodies work well in Western blot and electrophoretic mobility (gel shift) assays for identifying PSE1 or Ku. They may be used to immuno-deplete Ku protein from protein mixtures prior to specific DNA affinity chromatography.

Storage Conditions

These antibodies are stable for at least one (1) year at -20°C to -70°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.

Product Datasheet

https://www.qedbio.com/wp-content/uploads/pdf/70105.pdf

Specificity

70104 recognizes the small b subunit (~70 kDa) and 70105 recognizes the large alpha subunit (~80-90 kDa) of placental PSE1 and human Ku in solution and when bound to DNA.

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

CAS Number

9007-83-4

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