Set of 4 Chlamydomonas anti-Lhc antibodies
Selected Variant
AS01 011 CHLAMYset
Size: 50 µL
Product Specifications
Background
The outer light-harvesting antenna of the photosystems (PSI and PSII) of the green unicellular alga Chlamydomonas reinhardtii is composed of pigment-binding proteins belonging to the Lhc family highly conserved in photosynthetic eukaryotes. Some of the Lhc-subtypes of Chlamydomonas share sufficient high similarity with the respective functional homologs in plants to allow specific detection with antisera generated against conserved peptide domains from plant Lhc-proteins.
CAS Number
9007-83-4
HS Code
38221900
Gene ID
826479
Host
Rabbit
Reactivity
Chlamydomonas reinhardtii
Not reactive in
Lhcb4 - higher plants (use AS04 045 for those organsims), algae, cyanobacteria
Immunogen
Lhcb2 - BSA-conjugated peptideLhcb4 - KLH-conjugated synthetic peptide derived from Lhcb4 (CP29, Lhcbm4) protein sequence from Chlamydomonas reinhardtii (Q93WD2) Lhcb5 - KLH-conjugated synthetic peptide derived from Chlamydomonas reinhardtii Lhcb5 protein sequence (Q9FEK6) Lhcbm5 - SDS-PAGE purified polypeptide from Chlamydomonas reinhardtii LHCII-type II-enriched fractions
Clonality
Polyclonal
Applications
Western blot (WB)
Field of Research
LHC
Dilution
1 : 5000 (Lhcb2, Lhcb5, Lhcbm5) (WB) 1 : 10 000 (Lhcb4) (WB)
Purity
Total IgG. Protein G purified in PBS pH 7.4. (Lhcb2), serum (Lhcb4, Lhcb5, Lhcbm5) .
Format
Lyophilized
Molecular Weight
25 kDa (Lhcb2) 29 kDa (Lhcb4) 29 | 30 kDa (Lhcbm5) 30 | 26 kDa (Lhcb5)
References & Citations
Lhcb2 Du et al. (2018) . Galactoglycerolipid Lipase PGD1 Is Involved in Thylakoid Membrane Remodeling in Response to Adverse Environmental Conditions in Chlamydomonas. Plant Cell. 2018 Feb;30 (2) :447-465. doi: 10.1105/tpc.17.00446. Lhcb4Jeong et al. (2017) . Deletion of the chloroplast LTD protein impedes LHCI import and PSI-LHCI assembly in Chlamydomonas reinhardtii. J Exp Bot. 2017 Dec 30. doi: 10.1093/jxb/erx457. Lhcb5 and Lhcbm5 Takahashi et al. (2006) . Identification of the mobile light-harvesting complex II polypeptides for state transitions in Chlamydomonas reinhardtii. PNAS 103:477-482
Storage Conditions
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Prediction Reactivity
Algae (Lhcb2), Angiosperms, Dictos, Gymnosperms, Mosses
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