Recombinant Mouse BGLAP Protein (Trx Tag)

Product Specifications

Background

This locus represents naturally occurring read-through transcription between the neighboring PMF1 (polyamine-modulated factor 1) and BGLAP (bone gamma-carboxyglutamate Gla protein) genes on chromosome 1. Alternative splicing results in multiple transcript variants encoding isoforms that share sequence identity with the upstream gene product, but they contain distinct C-termini due to frameshifts versus the downstream gene coding sequence. Recombinant human PMF1-BGLAP protein, fused to His-tag at N-terminus, was expressed in E.coli.

Abbreviation

BGLAP

Synonyms

Bone Gamma-carboxyglutamate Protein; OT; Osteocalcin; BGP; Bglap; Bone Gla protein; Gamma-carboxyglutamic acid-containing protein

UniProt

P86546

Accession Number

P86546

Expression System

E.coli

Tag

N-Trx

Sequence

Tyr50-Ile95

Field of Research

Metabolism; Tags and Cell Markers; Developmental Biology

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.