Z-VDVAD-AFC

Z-VDVAD-AFC is a fluorogenic substrate. Z-VDVAD-AFC is used to measure the activity of cysteine protease 3 (Caspase-3) . Z-VDVAD-AFC undergoes hydrolysis to release 7-amino-4-trifluoromethylcoumarin (AFC) . AFC is fluorescent under ultraviolet light and can emit fluorescent signals[1].

Product Specifications

CAS Number

[219138-08-6]

UNSPSC

12352209

Target

Fluorescent Dye

Type

Peptides

Related Pathways

Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/z-vdvad-afc.html

Solubility

10 mM in DMSO

Smiles

FC(F)(C(C1=CC=C(NC([C@H](CC(O)=O)NC([C@H](C)NC([C@H](C(C)C)NC([C@H](CC(O)=O)NC([C@H](C(C)C)NC(OCC2=CC=CC=C2)=O)=O)=O)=O)=O)=O)C=C1O3)=CC3=O)F

Molecular Formula

C39H45F3N6O13

Molecular Weight

862.80

References & Citations

[1]Pallepati P, et, al. Mild thermotolerance induced at 40°C protects HeLa cells against activation of death receptor-mediated apoptosis by hydrogen peroxide. Free Radic Biol Med. 2011 Mar 15;50 (6) :667-79.|[2]Pallepati P, et, al. Mild thermotolerance induced at 40°C protects HeLa cells against activation of death receptor-mediated apoptosis by hydrogen peroxide. Free Radic Biol Med. 2011 Mar 15;50 (6) :667-79.