Hexa-His

Hexa-His (6X His Tag) is a commonly used affinity tag made up of six histidine residues. HEXA-HIS can bind to affinity chromatography media containing transition metal ions like nickel (Ni2+) or cobalt (Co2+), making it useful for protein purification[1][2].

Product Specifications

CAS Number

[64134-30-1]

Product Name Alternative

6X His Tag

UNSPSC

12352209

Hazard Statement

H302, H315, H319

Target

Biochemical Assay Reagents

Type

Peptides

Related Pathways

Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/Hexa-His.html

Concentration

10mM

Purity

99.84

Solubility

H2O : 50 mg/mL (ultrasonic)

Smiles

O=C(O)[C@H](CC1=CNC=N1)NC([C@H](CC2=CNC=N2)NC([C@H](CC3=CNC=N3)NC([C@H](CC4=CNC=N4)NC([C@H](CC5=CNC=N5)NC([C@H](CC6=CNC=N6)N)=O)=O)=O)=O)=O

Molecular Formula

C36H44N18O7

Molecular Weight

840.85

Precautions

H302, H315, H319

References & Citations

[1]Watly J, et al. Insight into the coordination and the binding sites of Cu (2+) by the histidyl-6-tag using experimental and computational tools. Inorg Chem. 2014 Jul 7;53 (13) :6675-83.|[2]Ana Paula de Mattos Arêas, et al. Synthesis of cholera toxin B subunit gene: cloning and expression of a functional 6XHis-tagged protein in Escherichia coli.Protein Expr Purif.2002 Aug;25 (3) :481-7.