TMB
Product Specifications
UNSPSC Description
TMB is a non-toxic, non-mutagenic, colorless dye that is also used as a chromogenic agent in enzyme detection systems based on horseradish peroxidase (HRP). TMB can function as a photoacoustic probe for reactive oxygen and nitrogen species (ROS/RNS), reacting with hypochlorite, hydrogen peroxide, singlet oxygen, and nitrogen dioxide to produce blue oxidation products. TMB can detect the singlet oxygen produced by the outer membrane of Escherichia coli ATCC 25922[1][2].
Target Antigen
Fluorescent Dye; Reactive Oxygen Species
Type
Dye Reagents
Related Pathways
Immunology/Inflammation;Metabolic Enzyme/Protease;NF-κB;Others
Field of Research
Others
Assay Protocol
https://www.medchemexpress.com/TMB.html
Solubility
DMSO : 25 mg/mL (ultrasonic)
Smiles
NC1=C(C)C=C(C2=CC(C)=C(N)C(C)=C2)C=C1C
Molecular Weight
240.34
References & Citations
[1]Bresolí-Obach R, et al. Tetramethylbenzidine: An Acoustogenic Photoacoustic Probe for Reactive Oxygen Species Detection. Sensors (Basel). 2020 Oct 21;20(20):5952. |[2]Frey A, et al. A stable and highly sensitive 3,3',5,5'-tetramethylbenzidine-based substrate reagent for enzyme-linked immunosorbent assays. J Immunol Methods. 2000 Jan 13;233(1-2):47-56.
Shipping Conditions
Room Temperature
Product Datasheet
http://file.medchemexpress.com/batch_PDF/HY-15930/TMB-DataSheet-MedChemExpress.pdf
Product MSDS
http://file.medchemexpress.com/batch_PDF/HY-15930/TMB-SDS-MedChemExpress.pdf
Clinical Information
No Development Reported
CAS Number
54827-17-7
Available Sizes
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