TMB

TMB is a non-toxic, non-mutagenic, colorless dye that is also used as a chromogenic agent in enzyme detection systems based on horseradish peroxidase (HRP) . TMB can function as a photoacoustic probe for reactive oxygen and nitrogen species (ROS/RNS), reacting with hypochlorite, hydrogen peroxide, singlet oxygen, and nitrogen dioxide to produce blue oxidation products. TMB can detect the singlet oxygen produced by the outer membrane of Escherichia coli ATCC 25922[1][2].

Product Specifications

CAS Number

[54827-17-7]

Product Name Alternative

BM blue; Sure Blue TMB

UNSPSC

12171500

Hazard Statement

H302, H315, H319, H335

Target

Fluorescent Dye; Reactive Oxygen Species (ROS)

Type

Dye Reagents

Related Pathways

Immunology/Inflammation; Metabolic Enzyme/Protease; NF-κB; Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/TMB.html

Purity

99.95

Solubility

DMSO : 25 mg/mL (ultrasonic)

Smiles

NC1=C(C)C=C(C2=CC(C)=C(N)C(C)=C2)C=C1C

Molecular Formula

C16H20N2

Molecular Weight

240.34

Precautions

H302, H315, H319, H335

References & Citations

[1]Bresolí-Obach R, et al. Tetramethylbenzidine: An Acoustogenic Photoacoustic Probe for Reactive Oxygen Species Detection. Sensors (Basel) . 2020 Oct 21;20 (20) :5952. |[2]Frey A, et al. A stable and highly sensitive 3,3',5,5'-tetramethylbenzidine-based substrate reagent for enzyme-linked immunosorbent assays. J Immunol Methods. 2000 Jan 13;233 (1-2) :47-56.