Methyl Green

Methyl Green is a non-intercalating fluorescent labeling agent that selectively binds to the major groove of DNA. Methyl Green electrostatically interacts with the major groove of DNA through positively charged groups, exhibiting key activities such as high affinity, resistance to photobleaching, and stable fluorescence emission. Methyl Green can be directly measured by microscopy and flow cytometry, with peaks at 633 and 677 nm. Methyl Green can be used for fluorescent labeling of the nuclei of embryonic tissues or cells, or DNA staining and cell activity detection in gel electrophoresis[1][2][3].

Product Specifications

CAS Number

[14855-76-6]

UNSPSC

12171500

Hazard Statement

H302, H318, H351, H361, H373, H410

Target

DNA Stain

Type

Dye Reagents

Related Pathways

Cell Cycle/DNA Damage

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/methyl-green.html

Solubility

DMSO : 125 mg/mL (ultrasonic)

Smiles

C[N+](C)(CC)C1=CC=C(/C(C2=CC=C(N(C)C)C=C2)=C3C=C/C(C=C\3)=[N+](C)\C)C=C1.[Br-].[Cl-]

Molecular Formula

C27H35BrClN3

Molecular Weight

516.94

Precautions

H302, H318, H351, H361, H373, H410

References & Citations

[1]Prieto D, et al. Application of the DNA-specific stain methyl green in the fluorescent labeling of embryos. J Vis Exp. 2015 May 2; (99) :e52769.|[2]Prieto D, et, al. A fast, low cost, and highly efficient fluorescent DNA labeling method using methyl green. Histochem Cell Biol. 2014 Sep;142 (3) :335-45.|[3]Kim SK, et al. Methyl green. A DNA major-groove binding drug. FEBS Lett. 1993 Jan 2;315 (1) :61-4.