Anti-Hu GAPDH Purified

GAPDH (glyceraldehyde-3-phosphate dehydrogenase) is an intracellular homotetramerizing enzyme, which catalyzes oxidation of glyceraldehyde-3-phosphate into 1,3-bisphosphoglycerate during glycolysis, and has also functions in DNA replication, gene expression, and microtubule bundling. As it binds to many proteins, its functions are very broad. GAPDH plays also roles in cancer progression, apoptosis, Huntington disease and Alzheimer´s disease.

Product Specifications

Certification

RUO

Negative Species

Mouse,Pig

Reactivity

Human

Immunogen

Human CD4-positive lymphocytes

Target Antigen

GAPDH

Clone

FF26A

Applications

WB, IP, IHC-F

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Liu Y, Cao J, Zhu YN, Ma Y, Murtaza G, Li Y, Wang JH, Pu YS: C1222C deletion in exon 8 of ABL1 is involved in carcinogenesis and cell cycle control of colorectal cancer through IRS1/PI3K/Akt pathway. Front Oncol. 2020 Aug 11;10:1385., URL: https://pubmed.ncbi.nlm.nih.gov/32850446/, *Tarantelli C, Cannas E, Ekeh H, Moscatello C, Gaudio E, Cascione L, Napoli S, Rech C, Testa A, Maniaci C, Rinaldi A, Zucca E, Stathis A, Ciulli A, Bertoni F: The bromodomain and extra-terminal domain degrader MZ1 exhibits preclinical anti-tumoral activity in diffuse large B-cell lymphoma of the activated B cell-like type. Explor Target Antitumor Ther. 2021;2 (6) :586-601., URL: https://pubmed.ncbi.nlm.nih.gov/36046113/, *Arkhypov I, Özbay Kurt FG, Bitsch R, Novak D, Petrova V, Lasser S, Hielscher T, Groth C, Lepper A, Hu X, Li W, Utikal J, Altevogt P, Umansky V: HSP90α induces immunosuppressive myeloid cells in melanoma via TLR4 signaling. J Immunother Cancer. 2022 Sep;10 (9) :e005551. , URL: https://pubmed.ncbi.nlm.nih.gov/36113897/, *Veggiani G, Villaseñor R, Martyn GD, Tang JQ, Krone MW, Gu J, Chen C, Waters ML, Pearce KH, Baubec T, Sidhu SS: High-affinity chromodomains engineered for improved detection of histone methylation and enhanced CRISPR-based gene repression. Nat Commun. 2022 Nov 30;13 (1) :7381., URL: https://pubmed.ncbi.nlm.nih.gov/36450737/, *Liu CC, Leclair P, Pedari F, Vieira H, Monajemi M, Sly LM, Reid GS, Lim CJ: Integrins and ERp57 coordinate to regulate cell surface calreticulin in immunogenic cell death. Front Oncol. 2019 May 28;9:411., URL: https://pubmed.ncbi.nlm.nih.gov/31192123/, *Fonseka CL, Hardman CS, Woo J, Singh R, Nahler J, Yang J, Chen YL, Kamaladasa A, Silva T, Salimi M, Gray N, Dong T, Malavige GN, Ogg GS: Dengue virus co-opts innate type 2 pathways to escape early control of viral replication. Commun Biol. 2022 Jul 22;5 (1) :735., URL: https://pubmed.ncbi.nlm.nih.gov/35869167/, *Hartwig J, Loebel M, Steiner S, Bauer S, Karadeniz Z, Roeger C, Skurk C, Scheibenbogen C, Sotzny F: Metformin attenuates ROS via FOXO3 activation in immune cells. Front Immunol. 2021 Apr 19;12:581799., URL: https://pubmed.ncbi.nlm.nih.gov/33953705/, *Triguero-Martínez A, Roy-Vallejo E, Montes N, de la Fuente H, Ortiz AM, Castañeda S, González-Álvaro I, Lamana A: Genetic LGALS1 variants are associated with heterogeneity in galectin-1 serum levels in patients with early arthritis. Int J Mol Sci. 2022 Jun 28;23 (13) :7181. , URL: https://pubmed.ncbi.nlm.nih.gov/35806182/

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The mouse monoclonal antibody FF26A recognizes human GAPDH (glyceraldehyne-3-phosphate dehydrogenase), a ubiquitously expressed 37 kDa enzyme involved in glycolysis and other processes.

Applications Notes

Western blotting: 1 µg/ml; both reducing and non-reducing conditions; excellent with human antigen, negative results with murine and porcine material.