SingleFlowEx CD5 Alexa Fluor® 700

SingleFlowEx is fluorochrome-labeled monoclonal antibody single reagent for use in laboratory-developed multicolor flow cytometry tests in order to examine human clinical samples. SingleFlowEx can be used in various multicolor combinations. Laboratory shall establish the SingleFlowEx performance characteristics in conjunction with other reagents in normal and pathological samples.

Product Specifications

Certification

RUO (GMP)

Clone

L17F12

Applications

FC (QC tested)

Concentration

0.020 mg/mL

Format

Alexa Fluor® 700

Buffer

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Components

1 vial of monoclonal antibody reagent.The content (1 mL) is sufficient for min. 100 Tests.

References & Citations

*Dunphy CH, Tang W: The value of CD64 expression in distinguishing acute myeloid leukemia with monocytic differentiation from other subtypes of acute myeloid leukemia: a flow cytometric analysis of 64 cases. Arch Pathol Lab Med. 2007 May;131 (5) :748-54., URL: http://www.ncbi.nlm.nih.gov/pubmed/17488160 , *Gong JZ, Lagoo AS, Peters D, Horvatinovich J, Benz P, Buckley PJ: Value of CD23 determination by flow cytometry in differentiating mantle cell lymphoma from chronic lymphocytic leukemia/small lymphocytic lymphoma. Am J Clin Pathol. 2001 Dec;116 (6) :893-7., URL: http://www.ncbi.nlm.nih.gov/pubmed/11764079 , *McAlister MS, Davis B, Pfuhl M, Driscoll PC: NMR analysis of the N-terminal SRCR domain of human CD5: engineering of a glycoprotein for superior characteristics in NMR experiments. Protein Eng. 1998 Oct;11 (10) :847-53., URL: https://www.ncbi.nlm.nih.gov/pubmed/9862202, *Shuster JJ, Falletta JM, Pullen DJ, Crist WM, Humphrey GB, Dowell BL, Wharam MD, Borowitz M: Prognostic factors in childhood T-cell acute lymphoblastic leukemia: a Pediatric Oncology Group study. Blood. 1990 Jan 1;75 (1) :166-73., URL: https://www.ncbi.nlm.nih.gov/pubmed/1688495, *Engleman EG, Warnke R, Fox RI, Dilley J, Benike CJ, Levy R: Studies of a human T lymphocyte antigen recognized by a monoclonal antibody. Proc Natl Acad Sci U S A. 1981 Mar;78 (3) :1791-5., URL: https://www.ncbi.nlm.nih.gov/pubmed/7015346

Other References

*Freedman AS, Freeman G, Whitman J, Segil J, Daley J, Levine H, Nadler LM: Expression and regulation of CD5 on in vitro activated human B cells. Eur J Immunol. 1989 May;19 (5) :849-55. , URL: https://www.ncbi.nlm.nih.gov/pubmed/2472277?dopt=A, *Raman C: CD5, an important regulator of lymphocyte selection and immune tolerance. Immunol Res. 2002;26 (1-3) :255-63., URL: https://pubmed.ncbi.nlm.nih.gov/12403363/